Lumiscene is revolutionary water based, chemical blood detection method. Lumiscene helps to
visualize latent/ invisible (e.g. washed or removed) blood stains and patterns through a chemiluminescence reaction and is specifically for use in crime scene forensic investigations. This chemical formula is based on luminol and fluorescein and has many superior qualities and advantages over classic luminol or tablet based luminol chemistry.
When a luminol solution comes in contact with blood the chemical luminescence emits dark blue light. When this emission spectrum is measured with a spectrum analyzer we see that the highest intensity is about 425 nanometers (nm).
Instead of emission at 425 nm. Lumiscene has its emission peak at 525 nm, when it comes in contact with blood. Thus, the “light” produced by the chemical luminescence of Lumiscene is light blue/green in color.
This emission of 525 nm benefits the naked eye visibility of latent bloodstains multiple times. Thus, in dark environments, the human eye is far more sensitive to the 525 nm. emission than to the emission of 425 nm.
The presence of fluorescein in the Lumiscene solution does not only change the emission spectra, it also acts as an enhancer of the chemiluminescence reaction and creates greater light intensity.
Most luminol solutions, whether tablet based or not, and fluorescein solutions contain hydrogen peroxide as an activator. Well known in the scientific community is the damaging effect of hydrogen peroxide on DNA. Since in forensic investigations the quality and/or quantity of the blood is not known, it is not recommended to apply a chemical solution with a high concentration of hydrogen peroxide. So the volume percentages of hydrogen peroxide should be kept to a minimum to minimize DNA damage.
The Lumiscene stock solution is activated by 2 calibrated hydrogen peroxide tablets, which will give the solution an extremely low hydrogen peroxide volume: less than 0.12% compared to the usual 3% and as shown in other substances and in over 800 tests, preformed much better than normal luminol solutions.
Lumiscene, like luminol, is sprayed in darkness and therefore it is difficult, if not impossible to see with the naked eye, to check if the complete investigated area coming into contact with the solution. When using Lumiscene the user can check the investigated area by using an alternate light source such as the BLUEMAXX-series of lights and orange goggles/filter. Where Lumiscene is sprayed, the dried droplets will fluoresce when examined by a forensic light source between 415 and 480 nm.
Lumiscene solution viewed under 455nm light and orange filter